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Cytogenetic characterization of Rhomboplites aurorubens and Ocyurus chrysurus, two monotypic genera of Lutjaninae from Cubagua Island, Venezuela, with a review of the cytogenetics of Lutjanidae (Teleostei: Perciformes) Neotropical Ichthyology
Nirchio,Mauro; Oliveira,Claudio; Ferreira,Daniela C.; Rondón,Rodolfo; Pérez,Julio E.; Hett,Anne Kathrin; Rossi,Anna Rita; Sola,Luciana.
Lutjanidae, commonly known as snappers, includes 105 species, grouped in four subfamilies. In spite of the high number of species and of its worldwide distribution, the family has been little investigated and the phylogenetic relationships among some of its genera and species are still cause for debate. Only a small number of the species has been cytogenetically analysed. This study reports the first description of the karyotype of Rhomboplites aurorubens as well as data concerning the distribution of the constitutive heterochromatin and the location of the 18S rRNA and the 5S rRNA genes. Specimens of Ocyurus chrysurus from Venezuela were also investigated for the same cytogenetic features. Both species have a 48 uniarmed karyotype, but R. aurorubens has a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-banding FISH; Karyotype NORs Ribosomal genes.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400005
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Cytogenetic studies in three species of Lutjanus (Perciformes: Lutjanidae: Lutjaninae) from the Isla Margarita, Venezuela Neotropical Ichthyology
Nirchio,Mauro; Rondón,Rodolfo; Oliveira,Claudio; Ferreira,Irani A.; Martins,Cesar; Pérez,Julio; Sola,Luciana; Rossi,Anna Rita.
In the present study, three species of Lutjaninae, Lutjanus analis, L. griseus and L. synagris, were analyzed by conventional Giemsa staining, C-banding and silver staining, to reveal active Nucleolus Organizer Regions (NORs). Fluorescent in situ hybridization (FISH) was also applied to establish the number and location of the ribosomal gene clusters (18S and 5S rRNA genes). Counts of diploid metaphasic cells revealed a diploid modal chromosome complement composed of 48 acrocentric chromosomes in both L. analis and L. griseus. Two cytotypes were observed in L. synagris: cytotype I, with 2n=48 acrocentric chromosomes, found in 19 specimens, and cytotype II, with 46 acrocentric chromosomes and one large metacentric, found in two specimens. The large...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Ribosomal genes; NOR polymorphism; C-banding; Robertsonian rearrangement.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100012
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Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family Neotropical Ichthyology
Hett,Anne Kathrin; Nirchio,Mauro; Oliveira,Claudio; Siccha,Zoila Raquel; Rossi,Anna Rita; Sola,Luciana.
This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-banding; COI; 5S rDNA; Mullet; NORs.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100008
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